Tool Talk with Thermo Fisher Scientific—Mar. 25

Enabling atomic resolution Cryogenic Electron Microscopy


Thursday, March 25, 2021
11 am – 12 pm EDT
Attendees can join via Zoom
Meeting ID: 945 8610 0937



Presented by Jeff Lengyel, Principal Scientist

While Cryo-EM was once thought of as a low resolution structural technique, the method is currently capable of generating nearly atomic resolution structures on a routine basis. The combination of Cryo-EM and other methods such as X-ray crystallography, nuclear magnetic resonance spectroscopy, and molecular dynamics modeling are allowing researchers to address scientific questions previously thought intractable.

Recently, Cryo-EM broke another scientific barrier by generating an atomic resolution reconstruction of Apoferritin to 1.22A (DOI: 10.1038/s41586-020-2829-0). This was enabled with new technological developments ranging from improved electron sources (EC-FEG), and improved detectors (Falcon 4 and Selectris Imaging Filter).

This presentation will discuss recent technological advancements in various aspects of Cryo-EM. These recent developments will not only enhance throughput, but also the resolution achieved with single particle analysis and cryo-electron tomography.